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KMID : 0380620070390030315
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Korean Journal of Food Science and Technology
2007 Volume.39 No. 3 p.315 ~ p.322
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Physiological Activity/Nutrition : Fibrinolytic, Immunostimulating, and Cytotoxic Activities of Microbial Strains Isolated from Kochujang
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Seo Mi-Young
Kim Seung-Ho
Lee Cheol-Ho
Cha Seong-Kwan
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Abstract
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his study was carried out to investigate the functional activities of microorganisms isolated from kochujang, such as fibrinolytic, immunostimulating, and cytotoxical activities, and to apply these microorganisms to kochujang products. Ninety-one microbial strains with proteolytic activity were selected from 294 strains isolated from traditional and commercial kochujang. Three strains (TPP 0014, TPP 6013, and TPP 6015) with high fibrinolytic activity were tested for their immunostimulating and cytotoxical activities. For the assessment of macrophage activation, cytokines such as tumor necrosis factor, interleukin-1α and nitrogen oxide were measured with the murine macrophage cell line RAW 264.7. In addition, the cytotoxical activities of the three strains were examined by MTT assay on the colon cancer cell line SNU-C4 and normal cell line CHO-K1. Using an API identifying kit, two of the microbial strains (TPP 0014 and TPP 6015) were identified as Bacillus stearothermophilus and the other strain (TPP 6013) was identified as B. amyloliquefacience.
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KEYWORD
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kochujang, fibrinolytic activity, immunostimulating activity, cytotoxical activity, Bacillus strains
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